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Adrenocorticotropic Hormone (ACTH)

Ordering Info & Methodology

Test Code

Cerner Synonyms


ACTH Level, ACTH, Adrenocorticotropic Hormone, Corticotropin, Adrenal Corticotropin Hormone

Turn Around Time


Analyzed within 4 days of receipt.

Sandwich immunoassay, electrochemiluminescence, Roche Cobas

Ordering Guide

ACTH. For petrosal sinus sampling, order ACTHPS battery.

Specimen Requirements

Sample Type

Collection Container

Alternate Collection Containers

EDTA Plasma


1.0 mL

Cannot be added on to previously collected sample.

Add on stability
This is the length of time after sample collection that this analyte is stable. Test can be added to a previously collected sample if the time since collection is less than the add on stability time.

Minimum Volume

Add On Stability

Collection and Shipping Requirements

Centrifuge at 4°C, freeze. Send frozen on dry ice. Must arrive at SPH frozen. Thawed specimens will be rejected.

Reference Intervals

Female Reference Interval

Age Category
Reference Interval
≥ 0y

Male Reference Interval

Age Category
Reference Interval
≥ 0y
Additional Notes

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Measurement Uncertainty (MU)
Every laboratory value is an estimate of the true concentration of substance. MU is the 95% confidence interval around that estimate. 95% of the time, the true concentration will be within the range of the reported value ±MU. This value is based on analytical variability (precision) and does not reflect other factors such as sample collection and handling which may also affect the result.


Reported sodium = 140 mmol/L
Sodium MU = 1%
Expanded uncertainty = 140 mmol/L ± 1% or 140mmol/L ± 1.4 mmol/L
Interpretation - the true value of sodium in the sample is in the range 138.6-141.4 mmol/L, with 95% confidence.


Measurement Uncertainty

Reference interval applies to specimens collected between 7 and 10 AM, or within the first 3 hours of waking. Assay interference is possible in the presence of elevated quantities of ACTH fragments, precursors and synthetic analogues. Assay interferences are possible in patients taking high dose (>5mg/day) biotin supplementation. 

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