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25-Hydroxyvitamin D

Ordering Info & Methodology

Test Code

Cerner Synonyms


Vitamin D 25-Hydroxy, Vit D, Vit D3, Vitamin D 25-Hydroxy, 25-Hydroxy D2, 25-Hydroxy D3, 25-Hydroxy Vitamin D, 25-Hydroxycholecalciferol, 25-OH Vitamin D, Calcidiol, D2, D3, Vitamin D Assay

Turn Around Time


Analyzed within 7 days of receipt.

Electrochemiluminecent immunoassay (routine); Liquid chromatography mass spectrometry (by request - contact lab)

Ordering Guide

Specimen Requirements

Sample Type

Collection Container

Alternate Collection Containers




1.0 mL

Add on stability
This is the length of time after sample collection that this analyte is stable. Test can be added to a previously collected sample if the time since collection is less than the add on stability time.

Minimum Volume

Add On Stability

Collection and Shipping Requirements

Freeze. Send on ice pack.

Reference Intervals

Female Reference Interval

Age Category
Reference Interval
≥0 y

Male Reference Interval

Age Category
Reference Interval
≥0 y
Additional Notes

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Measurement Uncertainty (MU)
Every laboratory value is an estimate of the true concentration of substance. MU is the 95% confidence interval around that estimate. 95% of the time, the true concentration will be within the range of the reported value ±MU. This value is based on analytical variability (precision) and does not reflect other factors such as sample collection and handling which may also affect the result.


Reported sodium = 140 mmol/L
Sodium MU = 1%
Expanded uncertainty = 140 mmol/L ± 1% or 140mmol/L ± 1.4 mmol/L
Interpretation - the true value of sodium in the sample is in the range 138.6-141.4 mmol/L, with 95% confidence.


Measurement Uncertainty

According to Canadian recommendations:

<25 nmol/L     Vitamin D deficiency 25-75 nmol/L  Suboptimal Vitamin D status >75 nmol/L     Desirable Vitamin D status >250 nmol/L   Potential Adverse effects


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