25-Hydroxyvitamin D
Ordering Info & Methodology
Test Code
Cerner Synonyms
VITD3
Vitamin D 25-Hydroxy, Vit D, Vit D3, Vitamin D 25-Hydroxy, 25-Hydroxy D2, 25-Hydroxy D3, 25-Hydroxy Vitamin D, 25-Hydroxycholecalciferol, 25-OH Vitamin D, Calcidiol, D2, D3, Vitamin D Assay
Turn Around Time
Methodology
Analyzed within 7 days of receipt.
Electrochemiluminecent immunoassay (routine); Liquid chromatography mass spectrometry (by request - contact lab)
Ordering Guide
Specimen Requirements
Sample Type
Collection Container
Alternate Collection Containers
Serum
Gold
Red
1.0 mL
Add on stability
This is the length of time after sample collection that this analyte is stable. Test can be added to a previously collected sample if the time since collection is less than the add on stability time.
Minimum Volume
Add On Stability
Collection and Shipping Requirements
Freeze. Send on ice pack.
Reference Intervals
Female Reference Interval
Age Category | Reference Interval | units |
|---|---|---|
≥0 y | 75-200 | nmol/L |
Male Reference Interval
Age Category | Reference Interval | units |
|---|---|---|
≥0 y | 75-200 | nmol/L |
Additional Notes
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Measurement Uncertainty (MU)
Every laboratory value is an estimate of the true concentration of substance. MU is the 95% confidence interval around that estimate. 95% of the time, the true concentration will be within the range of the reported value ±MU. This value is based on analytical variability (precision) and does not reflect other factors such as sample collection and handling which may also affect the result.
Example
Reported sodium = 140 mmol/L
Sodium MU = 1%
Expanded uncertainty = 140 mmol/L ± 1% or 140mmol/L ± 1.4 mmol/L
Interpretation - the true value of sodium in the sample is in the range 138.6-141.4 mmol/L, with 95% confidence.
8%
Measurement Uncertainty
According to Canadian recommendations:
<25 nmol/L Vitamin D deficiency 25-75 nmol/L Suboptimal Vitamin D status >75 nmol/L Desirable Vitamin D status >250 nmol/L Potential Adverse effects